Siyuan (Steven) Wang, PhD

Wang’s independent lab at Yale is devoted to understand mammalian genome architectures and spatial transcriptome in health and disease. In the past few years, the lab introduced a new integrative technique, termed Multiplexed Imaging of Nucleome Architectures (MINA) – the first comprehensive 3D nucleomic imaging technique (bioRxiv 2019; Nat Comm 2020; Nat Protoc 2021). This method enabled multi-omic and multiscale visualization of single-cell nucleome architectures and gene expression to functionally define promoter-enhancer interactions, chromatin domains, compartments, territories, and chromatin interactions with nuclear landmarks in the same single cells of complex mammalian tissues, generating true 3D maps of all major chromatin architectures reported by various sequencing methods (P-E loops, TADs, LADs, NADs, A-B compartments, and chromosome territories). In applying the technique to mouse fetal liver, the team discovered cell-type-specific chromatin architectures associated with gene expression as well as chromatin organization principles independent of cell type (bioRxiv 2019; Nat Comm 2020). They also applied chromatin tracing to elucidate novel architectures and their regulation in the folding conformations of the two copies of X chromosomes in female human cells (Genome Biology 2021; Science Advances 2023). Overall, the chromatin tracing and MINA technologies have revolutionized 3D genomics and multi-omics studies (Trends in Cell Biology “Best of 2021”, Nature Reviews Methods Primers 2024).